First-generation (1G) Bio-Ethanol is produced from biomass such as sugar containing materials (like sugar cane juice, molasses, sugar beet etc.) and starch containing materials (such as corn, cassava, rotten vegetables such as potatoes, damaged food grain etc.). Ethanol is generally produced from the fermentation of C5 and C6 sugars (mostly xylose and glucose) using classical or GMO yeast strains such as Saccharomyces cerevisiae.